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Anti-Human RAB18 Polyclonal Antibody (HP595014)

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Overview
Catalog No.HP595014
Description
Anti-Human RAB18 Polyclonal Antibody (HP595014) is a rabbit polyclonal antibody detecting RAB18 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Danio rerio.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human RAB18 (Met1-Lys183).
Target Ras-related protein Rab-18, RAB18
Purification Purified by antigen affinity column.
Accession Q9NP72
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Ras-related protein Rab-18 (RAB18) is a ~22 kDa protein. The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB18 is required for the localization of ZFYVE1 to lipid droplets and for its function in mediating the formation of endoplasmic reticulum-lipid droplets (ER-LD) contacts. Also required for maintaining endoplasmic reticulum structure. Plays a role in apical endocytosis/recycling.

1. Gerondopoulos, A. et al. (2014) The Journal of cell biology 205, 707-20. PMID: 24891604
2. Li, D. et al. (2019) Cell reports 27, 343-358.e5. PMID: 30970241
3. Bem, D. et al. (2011) American journal of human genetics 88, 499-507. PMID: 21473985
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human RAB18 Polyclonal Antibody

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