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Anti-Human RAB1B Polyclonal Antibody (HP116014)

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Overview
Catalog No.HP116014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human RAB1B (Met1-Gly176).
Target RAB1B, Ras-related protein Rab-1B
Purification Purified by antigen affinity column.
Accession Q9H0U4
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Ras-related protein Rab-1B (RAB1B) is a ~22 kDa protein. The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Plays a role in the initial events of the autophagic vacuole development which take place at specialized regions of the endoplasmic reticulum. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Required to modulate the compacted morphology of the Golgi.

1. Zoppino, FC. et al. (2010) Traffic (Copenhagen, Denmark) 11, 1246-61. PMID: 20545908
2. Overmeyer, JH. et al. (1998) Molecular biology of the cell 9, 223-35. PMID: 9437002
3. Gavriljuk, K. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 21348-53. PMID: 23236136
4. Aizawa, M. et al. (2015) The Journal of biological chemistry 290, 22250-61. PMID: 26209634
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human RAB1B Polyclonal Antibody

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