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Anti-Human RAB32 Polyclonal Antibody (HD207014)

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Overview
Catalog No.HD207014
Description
Anti-Human RAB32 Polyclonal Antibody (HD207014) is a rabbit polyclonal antibody detecting RAB32. Suitable for Human.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human RAB32 (Met1-Cys225).
Target EC:3.6.5.2, RAB32, Ras-related protein Rab-32
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q13637
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Ras-related protein Rab-32 (RAB32) is a ~24 kDa protein. The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Also acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A and anchoring it to the mitochondrion. Also involved in synchronization of mitochondrial fission. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.

1. Bao, X. et al. (2002) European journal of biochemistry 269, 259-71. PMID: 11784320
2. Nottingham, RM. et al. (2011) The Journal of biological chemistry 286, 33213-22. PMID: 21808068
3. Alto, NM. et al. (2002) The Journal of cell biology 158, 659-68. PMID: 12186851
4. Seto, S. et al. (2011) Traffic (Copenhagen, Denmark) 12, 407-20. PMID: 21255211
5. Gerondopoulos, A. et al. (2012) Current biology : CB 22, 2135-9. PMID: 23084991
6. Zhu, H. et al. (2023) Science (New York, N.Y.) 382, 1404-1411. PMID: 38127736
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-Human RAB32 Polyclonal Antibody

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