Anti-Human RAB3GAP1 Polyclonal Antibody (HC686014)

Name: Anti-Human RAB3GAP1 Polyclonal Antibody
Brand: abinScience
SKU: HC686014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HC686014

Description

Anti-Human RAB3GAP1 Polyclonal Antibody (HC686014) is a rabbit polyclonal antibody detecting RAB3GAP1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RAB3GAP1 (Met646-Asp756).

Target

Rab3 GTPase-activating protein catalytic subunit, RAB3 GTPase-activating protein 130 kDa subunit, Rab3-GAP p130, Rab3-GAP, RAB3GAP1, KIAA0066, RAB3GAP

Purification

Purified by antigen affinity column.

Accession

Q15042

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Rab3 GTPase-activating protein catalytic subunit (RAB3GAP1) is a ~110 kDa protein. Catalytic subunit of the Rab3 GTPase-activating (Rab3GAP) complex composed of RAB3GAP1 and RAB3GAP2, which accelerates the otherwise slow GTP hydrolysis catalyzed by Rab proteins. Has GTPase-activating protein (GAP) activity towards various Rab3 subfamily members (RAB3A, RAB3B, RAB3C and RAB3D), RAB5A and RAB43. Additionally, it has guanine nucleotide exchange factor (GEF) activity towards RAB18, promoting GDP release from RAB18 and the conversion of inactive RAB18-GDP to the active form RAB18-GTP. Recruits and stabilizes RAB18 at the cis-Golgi membrane in fibroblasts where RAB18 is most likely activated. Also involved in RAB18 recruitment at the endoplasmic reticulum (ER) membrane where it maintains proper ER structure.

1. Fukui, K. et al. (1997) The Journal of biological chemistry 272, 4655-8. PMID: 9030515

2. Clabecq, A. et al. (2000) The Journal of biological chemistry 275, 31786-91. PMID: 10859313

3. Fairlie, GMJ. et al. (2025) Nature communications 16, 479. PMID: 39779760

4. Gerondopoulos, A. et al. (2014) The Journal of cell biology 205, 707-20. PMID: 24891604

5. Handley, MT. et al. (2015) Open biology 5, 150047. PMID: 26063829

Note

For research use only.

Images

References

Datasheet

Anti-Human RAB3GAP1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human RAB3GAP1 Polyclonal Antibody (HC686014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us