Anti-Human RAB8A Polyclonal Antibody (HX163014)

Name: Anti-Human RAB8A Polyclonal Antibody
Brand: abinScience
SKU: HX163014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HX163014

Description

Anti-Human RAB8A Polyclonal Antibody (HX163014) is a rabbit polyclonal antibody detecting RAB8A in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Dog.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RAB8A (Lys21-Ser181).

Target

RAB8, MEL, Ras-related protein Rab-8A, Oncogene c-mel, RAB8A

Purification

Purified by antigen affinity column.

Accession

P61006

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ras-related protein Rab-8A (RAB8A) is a ~23 kDa protein. The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB8A is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Regulates the compacted morphology of the Golgi.

1. Bryant, DM. et al. (2010) Nature cell biology 12, 1035-45. PMID: 20890297

2. Aizawa, M. et al. (2015) The Journal of biological chemistry 290, 22250-61. PMID: 26209634

3. Roland, JT. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 2789-94. PMID: 21282656

4. Chen, Y. et al. (2012) Cell research 22, 333-45. PMID: 21844891

5. Dhekne, HS. et al. (2018) eLife 7. PMID: 30398148

6. Murga-Zamalloa, CA. et al. (2010) Human molecular genetics 19, 3591-8. PMID: 20631154

7. Sellier, C. et al. (2016) The EMBO journal 35, 1276-97. PMID: 27103069

8. Lucken-Ardjomande Häsler, S. et al. (2020) The Journal of cell biology 219. PMID: 32344433

9. Eguchi, T. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E9115-E9124. PMID: 30209220

Note

For research use only.

Images

References

Datasheet

Anti-Human RAB8A Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human RAB8A Polyclonal Antibody (HX163014)

Overview

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References

Datasheet

SDS

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