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Anti-Human RABGEF1 Polyclonal Antibody (HP693014)

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Overview
Catalog No.HP693014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human RABGEF1 (Thr131-Lys368).
Target RAP1, Rabex-5, RABEX5, Rab5 GDP/GTP exchange factor, RABGEF1, Rabaptin-5-associated exchange factor for Rab5
Purification Purified by antigen affinity column.
Accession Q9UJ41
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Rab5 GDP/GTP exchange factor (RABGEF1) is a ~56 kDa protein. Acts as a guanine-nucleotide releasing factor (GEF) for RAB5A by promoting the conversion of inactive RAB-GDP to the active form RAB-GTP. Forms a complex with RABEP1 that acts as Rab4/Rab5 effector regulating endosome fusion. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Also functions as a ubiquitin ligase.

1. Delprato, A. et al. (2004) Cell 118, 607-17. PMID: 15339665
2. Horiuchi, H. et al. (1997) Cell 90, 1149-59. PMID: 9323142
3. Lippé, R. et al. (2001) Molecular biology of the cell 12, 2219-28. PMID: 11452015
Note For research use only.
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Formula
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Anti-Human RABGEF1 Polyclonal Antibody

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