Anti-Human RACK1 Polyclonal Antibody (HX977014)

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References
Datasheet
SDS

Overview

Catalog No.

HX977014

Description

Anti-Human RACK1 Polyclonal Antibody (HX977014) is a rabbit polyclonal antibody detecting RACK1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RACK1 (Met1-Arg317).

Target

RACK1, Human lung cancer oncogene 7 protein, Guanine nucleotide-binding protein subunit beta-like protein 12.3, GNB2L1, Cell proliferation-inducing gene 21 protein, Guanine nucleotide-binding protein subunit beta-2-like 1, Receptor of activated protein C kinase 1, Guanine nucleotide-binding protein subunit beta-2-like 1, N-terminally processed, Receptor for activated C kinase, Small ribosomal subunit protein RACK1, HLC-7

Purification

Purified by antigen affinity column.

Accession

P63244

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Small ribosomal subunit protein RACK1 is a ~35 kDa protein. Scaffolding protein involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression. Involved in the initiation of the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation, by promoting ubiquitination of a subset of 40S ribosomal subunits. Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation.

1. Anger, AM. et al. (2013) Nature 497, 80-5. PMID: 23636399

2. Sundaramoorthy, E. et al. (2017) Molecular cell 65, 751-760.e4. PMID: 28132843

3. Ikebuchi, Y. et al. (2009) Hepatology research : the official journal of the Japan Society of Hepatology 39, 1091-107. PMID: 19674157

4. Cao, XX. et al. (2011) Breast cancer research and treatment 126, 555-63. PMID: 20499158

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human RACK1 polyclonal antibody at 1ug/mL on recombinant Human RACK1 (Catalog No. HX977012).

References

Datasheet

Anti-Human RACK1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human RACK1 Polyclonal Antibody (HX977014)

Overview

Images

References

Datasheet

SDS

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