Anti-Human RAD17 Polyclonal Antibody (HF857014)

Name: Anti-Human RAD17 Polyclonal Antibody
Brand: abinScience
SKU: HF857014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HF857014

Description

Anti-Human RAD17 Polyclonal Antibody (HF857014) is a rabbit polyclonal antibody detecting RAD17 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RAD17 (Lys96-Pro353).

Target

Cell cycle checkpoint protein RAD17, hRad17, RF-C/activator 1 homolog, RAD17, R24L

Purification

Purified by antigen affinity column.

Accession

O75943

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cell cycle checkpoint protein RAD17 is a ~77 kDa protein. Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the 9-1-1 (RAD1-RAD9-HUS1) complex and RHNO1 onto chromatin, and in CHEK1 activation. Involved in homologous recombination by mediating recruitment of the MRN complex to DNA damage sites. May also serve as a sensor of DNA replication progression.

1. Li, L. et al. (1999) Oncogene 18, 1689-99. PMID: 10208430

2. Bao, S. et al. (2001) Nature 411, 969-74. PMID: 11418864

3. Post, S. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 13102-7. PMID: 11687627

4. Zou, L. et al. (2002) Genes & development 16, 198-208. PMID: 11799063

5. Wang, X. et al. (2003) Genes & development 17, 965-70. PMID: 12672690

6. Ellison, V. et al. (2003) PLoS biology 1, E33. PMID: 14624239

7. Garg, R. et al. (2004) Molecular cancer research : MCR 2, 362-9. PMID: 15235112

8. Cotta-Ramusino, C. et al. (2011) Science (New York, N.Y.) 332, 1313-7. PMID: 21659603

10. Bermudez, VP. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 1633-8. PMID: 12578958

Note

For research use only.

Images

References

Datasheet

Anti-Human RAD17 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human RAD17 Polyclonal Antibody (HF857014)

Overview

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References

Datasheet

SDS

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