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Anti-Human RAPGEF1 Polyclonal Antibody (HC394014)

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Overview
Catalog No.HC394014
Description
Anti-Human RAPGEF1 Polyclonal Antibody (HC394014) is a rabbit polyclonal antibody detecting RAPGEF1. Suitable for Human.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human RAPGEF1 (His842-Thr1077).
Target CRK SH3-binding GNRP, GRF2, Guanine nucleotide-releasing factor 2, Protein C3G, RAPGEF1, Rap guanine nucleotide exchange factor 1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q13905
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Rap guanine nucleotide exchange factor 1 (RAPGEF1) is a ~120 kDa protein. Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth.

1. Sakakibara, A. et al. (2002) Journal of cell science 115, 4915-24. PMID: 12432078
Note For research use only
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Formula
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Formula
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V₁ (Stock Vol.)
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C₂ (Working Conc.)
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Anti-Human RAPGEF1 Polyclonal Antibody

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