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Anti-Human RCC2 Polyclonal Antibody (HA217014)

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Overview
Catalog No.HA217014
Description
Anti-Human RCC2 Polyclonal Antibody (HA217014) is a rabbit polyclonal antibody detecting Protein RCC2 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human RCC2 (Glu89-Arg501).
Target Protein RCC2, Telophase disk protein of 60 kDa, RCC2, RCC1-like protein TD-60, KIAA1470, TD60
Purification Purified by antigen affinity column.
Accession Q9P258
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Protein RCC2 is a ~56 kDa protein. Multifunctional protein that may affect its functions by regulating the activity of small GTPases, such as RAC1 and RALA. Required for normal progress through the cell cycle, both during interphase and during mitosis. Required for the presence of normal levels of MAD2L1, AURKB and BIRC5 on inner centromeres during mitosis, and for normal attachment of kinetochores to mitotic spindles. Required for normal organization of the microtubule cytoskeleton in interphase cells. Functions as guanine nucleotide exchange factor (GEF) for RALA.

1. Mollinari, C. et al. (2003) Developmental cell 5, 295-307. PMID: 12919680
2. Williamson, RC. et al. (2014) Journal of cell science 127, 4292-307. PMID: 25074804
3. Papini, D. et al. (2015) Nature communications 6, 7678. PMID: 26158537
4. Song, C. et al. (2018) Oncogene 37, 8-17. PMID: 28869598
5. Yenjerla, M. et al. (2013) Cell cycle (Georgetown, Tex.) 12, 837-41. PMID: 23388455
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human RCC2 Polyclonal Antibody

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