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Anti-Human RFX5 Polyclonal Antibody (HD042014)

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Anti-Human RFX5 Polyclonal Antibody
Anti-Human RFX5 Polyclonal Antibody
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  • 100ug
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  • Datasheet

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Overview
Catalog No.HD042014
Description
Anti-Human RFX5 Polyclonal Antibody (HD042014) is a rabbit polyclonal antibody detecting RFX5. Suitable for Mouse and Human.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human RFX5 (Glu26-Gly250).
Target DNA-binding protein RFX5, RFX5, Regulatory factor X 5
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P48382
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

DNA-binding protein RFX5 is a ~65 kDa protein. Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters.

Note For research use only
Images
  • Anti-Human RFX5 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-RFX5 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: K562 cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human RFX5 Polyclonal Antibody

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