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Anti-Human RFXANK Polyclonal Antibody (HT356014)

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Overview
Catalog No.HT356014
Description
Anti-Human RFXANK Polyclonal Antibody (HT356014) is a rabbit polyclonal antibody detecting RFXANK in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human RFXANK (Ser91-Glu260).
Target DNA-binding protein RFXANK, Ankyrin repeat family A protein 1, Regulatory factor X subunit B, RFX-B, Regulatory factor X-associated ankyrin-containing protein, RFXANK, ANKRA1, RFXB
Purification Purified by antigen affinity column.
Accession O14593
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

DNA-binding protein RFXANK is a ~28 kDa protein. Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator/CIITA. May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters. May also potentiate the activation of RAF1.

1. Nagarajan, UM. et al. (1999) Immunity 10, 153-62. PMID: 10072068
2. Nagarajan, UM. et al. (2000) Journal of immunology (Baltimore, Md. : 1950) 164, 3666-74. PMID: 10725724
3. Masternak, K. et al. (1998) Nature genetics 20, 273-7. PMID: 9806546
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human RFXANK Polyclonal Antibody

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