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Anti-Human RHO/Rhodopsin Recombinant Antibody (K42-41L) (HY021013)

Anti-Human RHO/Rhodopsin Recombinant Antibody (K42-41L)
Anti-Human RHO/Rhodopsin Recombinant Antibody (K42-41L)
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Overview
Catalog No.HY021013
Species reactivityHuman
ApplicationsBlocking, ELISA, ICC
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDK42-41L
Clonality Monoclonal
Target Rhodopsin, OPN2, Opsin-2, RHO
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P08100
RRID

Anti-Human RHO/Rhodopsin Antibody (K42-41L) (abinScience Cat# HY021013, RRID:AB_3726901)
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Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Rhodopsin is a ~38 kDa protein. Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling.

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1. Sieving, PA. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 880-4. PMID: 7846071
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2. Rao, VR. et al. (1994) Nature 367, 639-42. PMID: 8107847
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3. Noorwez, SM. et al. (2003) The Journal of biological chemistry 278, 14442-14450. PMID: 12566452
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4. Dryja, TP. et al. (1990) The New England journal of medicine 323, 1302-7. PMID: 2215617
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5. Kang, Y. et al. (2015) Nature 523, 561-7. PMID: 26200343
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6. He, Y. et al. (2017) Cell research 27, 728-747. PMID: 28524165
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7. Zhou, XE. et al. (2017) Cell 170, 457-469.e13. PMID: 28753425
Note For research use only.
Images
  • Anti-Human RHO/Rhodopsin Recombinant Antibody (K42-41L)

    SDS-PAGE

    SDS-PAGE for Anti-Human RHO/Rhodopsin Antibody (K42-41L)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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