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Anti-Human RHOT2 Polyclonal Antibody (HP192014)

Anti-Human RHOT2 Polyclonal Antibody
Anti-Human RHOT2 Polyclonal Antibody
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Overview
Catalog No.HP192014
Description
Anti-Human RHOT2 Polyclonal Antibody (HP192014) is a rabbit polyclonal antibody detecting RHOT2 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human RHOT2 (Glu177-Phe589).
Target Ras homolog gene family member T2, MIRO-2, ARHT2, hMiro-2, Mitochondrial Rho GTPase 2, C16orf39, RHOT2
Purification Purified by antigen affinity column.
Accession Q8IXI1
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Mitochondrial Rho GTPase 2 (RHOT2) is a ~68 kDa protein. Atypical mitochondrial nucleoside-triphosphatase (NTPase) involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution. Can hydrolyze GTP. Can hydrolyze ATP and UTP.

1. Fransson, S. et al. (2006) Biochemical and biophysical research communications 344, 500-10. PMID: 16630562
2. Liu, S. et al. (2012) PLoS genetics 8, e1002537. PMID: 22396657
3. Peters, DT. et al. (2018) International journal of molecular sciences 19. PMID: 30513825
Note For research use only.
Images
  • Anti-Human RHOT2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-RHOT2 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Hela cell lysate
    Lane 2: K562 cell lysate
    Lane 3: LO2 cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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