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Anti-Human RIOK1 Polyclonal Antibody (HV201014)

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Overview
Catalog No.HV201014
Description
Anti-Human RIOK1 Polyclonal Antibody (HV201014) is a rabbit polyclonal antibody detecting RIOK1. Suitable for Human.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human RIOK1 (Thr180-Val479).
Target EC:2.7.11.1, EC:3.6.1.-, RIO kinase 1, RIO1, RIOK1, Serine/threonine-protein kinase RIO1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q9BRS2
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Serine/threonine-protein kinase RIO1 (RIOK1) is a ~65 kDa protein. Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in processing of 18S-E pre-rRNA to the mature 18S rRNA. Required for the recycling of NOB1 and PNO1 from the late 40S precursor. The association with the very late 40S subunit intermediate may involve a translation-like checkpoint point cycle preceeding the binding to the 60S ribosomal subunit. Despite the protein kinase domain is proposed to act predominantly as an ATPase.

1. Widmann, B. et al. (2012) Molecular biology of the cell 23, 22-35. PMID: 22072790
2. Guderian, G. et al. (2011) The Journal of biological chemistry 286, 1976-86. PMID: 21081503
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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V₁ (Stock Vol.)
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C₂ (Working Conc.)
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Anti-Human RIOK1 Polyclonal Antibody

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