Anti-Human RNF13 Polyclonal Antibody (HT135014)

Name: Anti-Human RNF13 Polyclonal Antibody
Brand: abinScience
SKU: HT135014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HT135014

Description

Anti-Human RNF13 Polyclonal Antibody (HT135014) is a rabbit polyclonal antibody detecting RNF13 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human RNF13 (Glu33-Glu175).

Target

E3 ubiquitin-protein ligase RNF13, 2.3.2.27, RING finger protein 13, RNF13, RZF

Purification

Purified by antigen affinity column.

Accession

O43567

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

E3 ubiquitin-protein ligase RNF13 is a ~42 kDa protein. E3 ubiquitin-protein ligase that regulates cell proliferation. Involved in apoptosis regulation. Mediates ER stress-induced activation of JNK signaling pathway and apoptosis by promoting ERN1 activation and splicing of XBP1 mRNA. Also involved in protein trafficking and localization.

1. Zhang, Q. et al. (2009) Cell research 19, 348-57. PMID: 18794910

2. Arshad, M. et al. (2013) The Journal of biological chemistry 288, 8726-8736. PMID: 23378536

3. Edvardson, S. et al. (2019) American journal of human genetics 104, 179-185. PMID: 30595371

4. van Dijk, JR. et al. (2014) The Biochemical journal 459, 27-36. PMID: 24387786

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-RNF13 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse brain lysate
Lane 2: Rat brain lysate

References

Datasheet

Anti-Human RNF13 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human RNF13 Polyclonal Antibody (HT135014)

Overview

Images

References

Datasheet

SDS

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