Anti-Human ROCK1 Polyclonal Antibody (HC470014)

Name: Anti-Human ROCK1 Polyclonal Antibody
Brand: abinScience
SKU: HC470014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HC470014

Description

Anti-Human ROCK1 Polyclonal Antibody (HC470014) is a rabbit polyclonal antibody detecting ROCK1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human ROCK1 (Met1-Thr351).

Target

Renal carcinoma antigen NY-REN-35, p160 ROCK-1, p160ROCK, Rho-associated protein kinase 1, Rho-associated, coiled-coil-containing protein kinase I, Rho-associated, coiled-coil-containing protein kinase 1, ROCK-I, ROCK1

Purification

Purified by antigen affinity column.

Accession

Q13464

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Rho-associated protein kinase 1 (ROCK1) is a ~158 kDa protein. Protein kinase which is a key regulator of the actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability.

1. Maekawa, M. et al. (1999) Science (New York, N.Y.) 285, 895-8. PMID: 10436159

2. Ohashi, K. et al. (2000) The Journal of biological chemistry 275, 3577-82. PMID: 10652353

3. Sumi, T. et al. (2001) The Journal of biological chemistry 276, 670-6. PMID: 11018042

4. Sebbagh, M. et al. (2001) Nature cell biology 3, 346-52. PMID: 11283607

5. Hagerty, L. et al. (2007) The Journal of biological chemistry 282, 4884-4893. PMID: 17158456

6. Shao, J. et al. (2008) Molecular and cellular biology 28, 5196-208. PMID: 18573880

7. Wang, Y. et al. (2009) Circulation research 104, 531-40. PMID: 19131646

8. Ishizaki, T. et al. (1996) The EMBO journal 15, 1885-93. PMID: 8617235

9. Van Eyk, JE. et al. (1998) The Journal of biological chemistry 273, 23433-9. PMID: 9722579

Note

For research use only.

Images

References

Datasheet

Anti-Human ROCK1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human ROCK1 Polyclonal Antibody (HC470014)

Overview

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References

Datasheet

SDS

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