Anti-Human RPA2 Polyclonal Antibody (HB910014)

Name: Anti-Human RPA2 Polyclonal Antibody
Brand: abinScience
SKU: HB910014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB910014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RPA2 (Thr50-Glu270).

Target

RPA34, Replication protein A 34 kDa subunit, Replication protein A 32 kDa subunit, RF-A protein 2, RP-A p34, Replication factor A protein 2, REPA2, RPA2, RPA32, RP-A p32

Purification

Purified by antigen affinity column.

Accession

P15927

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Replication protein A 32 kDa subunit (RPA2) is a ~29 kDa protein. As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response.

1. Gupta, R. et al. (2007) Blood 110, 2390-8. PMID: 17596542

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-RPA2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: SH-SY5Y cell lysate
Lane 2: Hela cell lysate
Lane 3: Jurkat cell lysate
Lane 4: MOLT-4 cell lysate
Lane 5: THP-1 cell lysate
Lane 6: Lncap cell lystae
Lane 7: 293T cell lysate
Western blot

Western blot analysis was performed using anti-RPA2 polyclonal antibody at 1μg/ml on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: Hela cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Molt-4 cell lysate
Lane 5: THP-1 cell lysate
Lane 6: Lncap cell lysate
Lane 7: 293T cell lysate

References

Datasheet

Anti-Human RPA2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human RPA2 Polyclonal Antibody (HB910014)

Overview

Images

References

Datasheet

SDS

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