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Anti-Human RPA4 Polyclonal Antibody (HD580014)

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Overview
Catalog No.HD580014
Description
Anti-Human RPA4 Polyclonal Antibody (HD580014) is a rabbit polyclonal antibody detecting RPA4. Suitable for Human.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human RPA4 (Cys49-Asp261).
Target RF-A protein 4, RP-A p30, RPA4, Replication factor A protein 4, Replication protein A 30 kDa subunit
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q13156
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Replication protein A 30 kDa subunit (RPA4) is a ~28 kDa protein. As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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