Anti-Human RPL11 Polyclonal Antibody (HX025014)

Name: Anti-Human RPL11 Polyclonal Antibody
Brand: abinScience
SKU: HX025014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HX025014

Description

Anti-Human RPL11 Polyclonal Antibody (HX025014) is a rabbit polyclonal antibody detecting RPL11 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human RPL11 (Met1-Lys178).

Target

60S ribosomal protein L11, CLL-associated antigen KW-12, Large ribosomal subunit protein uL5, RPL11

Purification

Purified by antigen affinity column.

Accession

P62913

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Large ribosomal subunit protein uL5 (RPL11) is a ~20 kDa protein. Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs.

1. Cmejla, R. et al. (2009) Human mutation 30, 321-7. PMID: 19191325

2. Liang, X. et al. (2020) Nature communications 11, 3542. PMID: 32669547

3. Odintsova, TI. et al. (2003) Journal of protein chemistry 22, 249-58. PMID: 12962325

4. Gazda, HT. et al. (2008) American journal of human genetics 83, 769-80. PMID: 19061985

5. Sloan, KE. et al. (2013) Cell reports 5, 237-47. PMID: 24120868

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-RPL11 polyclonal antibody at 1ug/mL on various samples.
Lane 1: BXPC-3 cell lysate
Lane 2: A549 cell lysate
Lane 3: HepG2 cell lysate
Lane 5: NIH3T3 cell lysate
Lane 7: Mouse lung lysate
Lane 8: Mouse pancreas lysate
Lane 9: Rat liver lysate
Lane 10: Rat lung lysate
Lane11: Rat pancreas lysate

References

Datasheet

Anti-Human RPL11 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human RPL11 Polyclonal Antibody (HX025014)

Overview

Images

References

Datasheet

SDS

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