Anti-Human RPL23A Polyclonal Antibody (HX936014)

Name: Anti-Human RPL23A Polyclonal Antibody
Brand: abinScience
SKU: HX936014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HX936014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RPL23A (Lys70-Ile156).

Target

Large ribosomal subunit protein uL23, RPL23A, 60S ribosomal protein L23a

Purification

Purified by antigen affinity column.

Accession

P62750

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Large ribosomal subunit protein uL23 (RPL23A) is a ~17 kDa protein. Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Binds a specific region on the 26S rRNA. May promote p53/TP53 degradation possibly through the stimulation of MDM2-mediated TP53 polyubiquitination.

1. Anger, AM. et al. (2013) Nature 497, 80-5. PMID: 23636399

2. Liang, X. et al. (2020) Nature communications 11, 3542. PMID: 32669547

3. Datta, D. et al. (2015) The Journal of biological chemistry 290, 21536-52. PMID: 26203195

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-RPL23A polyclonal antibody at 1ug/mL on various samples.
Lane 1: 293T cell lysate
Lane 2: Jurkat cell lysate
Lane 3: U87 cell lysate
Lane 4: Mouse brain lysate
Lane 5: Mouse lung lysate
Lane 6: Mouse liver lysate
Lane 7: Rat brain lysate
Lane 8: Rat lung lysate
Lane 9: Rat liver lysate

References

Datasheet

Anti-Human RPL23A Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human RPL23A Polyclonal Antibody (HX936014)

Overview

Images

References

Datasheet

SDS

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