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Anti-Human RPP25 Polyclonal Antibody (HP449014)

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Overview
Catalog No.HP449014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human RPP25 (Met1-Ala199).
Target Ribonuclease P protein subunit p25, RPP25, RNase P protein subunit p25
Purification Purified by antigen affinity column.
Accession Q9BUL9
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Ribonuclease P protein subunit p25 (RPP25) is a ~20 kDa protein. Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.

1. Guerrier-Takada, C. et al. (2002) RNA (New York, N.Y.) 8, 290-5. PMID: 12003489
2. Welting, TJ. et al. (2006) RNA (New York, N.Y.) 12, 1373-82. PMID: 16723659
3. Wu, J. et al. (2018) Cell 175, 1393-1404.e11. PMID: 30454648
4. Goldfarb, KC. et al. (2017) Genes & development 31, 59-71. PMID: 28115465
Note For research use only.
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Formula
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Anti-Human RPP25 Polyclonal Antibody

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