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Anti-Human RPS27 Polyclonal Antibody (HC735014)

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Overview
Catalog No.HC735014
Description
Anti-Human RPS27 Polyclonal Antibody (HC735014) is a rabbit polyclonal antibody detecting RPS27. Suitable for Human, Rat, and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human RPS27 (Ser11-His84).
Target 40S ribosomal protein S27, MPS-1, MPS1, Metallopan-stimulin 1, RPS27, Small ribosomal subunit protein eS27
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P42677
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Small ribosomal subunit protein eS27 (RPS27) is a ~9 kDa protein. Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Required for proper rRNA processing and maturation of 18S rRNAs. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.

1. Anger, AM. et al. (2013) Nature 497, 80-5. PMID: 23636399
2. Vladimirov, SN. et al. (1996) European journal of biochemistry 239, 144-9. PMID: 8706699
3. Wang, R. et al. (2015) British journal of haematology 168, 854-64. PMID: 25424902
4. Singh, S. et al. (2021) Science (New York, N.Y.) 373, eabj5338. PMID: 34516797
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human RPS27 Polyclonal Antibody

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