Anti-Human RPS3 Polyclonal Antibody (HB915014)

Name: Anti-Human RPS3 Polyclonal Antibody
Brand: abinScience
SKU: HB915014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HB915014

Description

Anti-Human RPS3 Polyclonal Antibody (HB915014) is a rabbit polyclonal antibody detecting RPS3 in ELISA, IHC, WB. Suitable for Ambystoma mexicanum, Bovine, Dog, Human, and Ictalurus punctatus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RPS3 (Met1-Lys187).

Target

Small ribosomal subunit protein uS3, RPS3, 40S ribosomal protein S3

Purification

Purified by antigen affinity column.

Accession

P23396

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Small ribosomal subunit protein uS3 (RPS3) is a ~26 kDa protein. Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Has endonuclease activity and plays a role in repair of damaged DNA. Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA. Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS).

1. Anger, AM. et al. (2013) Nature 497, 80-5. PMID: 23636399

2. Vladimirov, SN. et al. (1996) European journal of biochemistry 239, 144-9. PMID: 8706699

3. Kim, J. et al. (1995) The Journal of biological chemistry 270, 13620-9. PMID: 7775413

4. Kim, SH. et al. (2005) Biochemical and biophysical research communications 328, 962-7. PMID: 15707971

5. Hegde, V. et al. (2004) DNA repair 3, 121-6. PMID: 14706345

6. Balyeva, KE. et al. (2008) Molekuliarnaia biologiia 42, 314-22. PMID: 18610840

7. Hegde, V. et al. (2004) Biochemistry 43, 14211-7. PMID: 15518571

8. Ko, SI. et al. (2008) Mutation research 648, 54-64. PMID: 18973764

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human RPS3 polyclonal antibody at 1ug/mL on recombinant Human RPS3 (Catalog No. HB915012).

References

Datasheet

Anti-Human RPS3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human RPS3 Polyclonal Antibody (HB915014)

Overview

Images

References

Datasheet

SDS

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