Anti-Human RUVBL2 Polyclonal Antibody (HP869014)

Name: Anti-Human RUVBL2 Polyclonal Antibody
Brand: abinScience
SKU: HP869014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HP869014

Description

Anti-Human RUVBL2 Polyclonal Antibody (HP869014) is a rabbit polyclonal antibody detecting RUVBL2 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Danio rerio, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RUVBL2 (Gln49-Ser463).

Target

48 kDa TATA box-binding protein-interacting protein, RuvB-like 2, INO80 complex subunit J, RUVBL2, TAP54-beta, Reptin 52, TIP60-associated protein 54-beta, TIP48, TIP49B, 51 kDa erythrocyte cytosolic protein, 48 kDa TBP-interacting protein, Repressing pontin 52, INO80J, TIP49b, ECP-51

Purification

Purified by antigen affinity column.

Accession

Q9Y230

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

RuvB-like 2 (RUVBL2) is a ~51 kDa protein. Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome -DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400.

1. Kanemaki, M. et al. (1999) The Journal of biological chemistry 274, 22437-44. PMID: 10428817

2. Puri, T. et al. (2007) Journal of molecular biology 366, 179-92. PMID: 17157868

3. López-Perrote, A. et al. (2020) eLife 9. PMID: 33205750

4. Doyon, Y. et al. (2004) Molecular and cellular biology 24, 1884-96. PMID: 14966270

5. Obri, A. et al. (2014) Nature 505, 648-53. PMID: 24463511

6. Jin, J. et al. (2005) The Journal of biological chemistry 280, 41207-12. PMID: 16230350

7. Chen, L. et al. (2011) The Journal of biological chemistry 286, 11283-9. PMID: 21303910

8. Wood, MA. et al. (2000) Molecular cell 5, 321-30. PMID: 10882073

Note

For research use only.

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References

Datasheet

Anti-Human RUVBL2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-Human RUVBL2 Polyclonal Antibody (HP869014)

Overview

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Datasheet

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