Anti-Human SAE1 Polyclonal Antibody (HA855014)

Name: Anti-Human SAE1 Polyclonal Antibody
Brand: abinScience
SKU: HA855014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HA855014

Description

Anti-Human SAE1 Polyclonal Antibody (HA855014) is a rabbit polyclonal antibody detecting SAE1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SAE1 (Met1-Lys346).

Target

SAE1, AOS1, Ubiquitin-like 1-activating enzyme E1A, SUA1, SUMO-activating enzyme subunit 1, UBLE1A

Purification

Purified by antigen affinity column.

Accession

Q9UBE0

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

SUMO-activating enzyme subunit 1 (SAE1) is a ~38 kDa protein. The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-SAE1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: NIH3T3 cell lysate
Lane 4: HT-29 cell lysate

References

Datasheet

Anti-Human SAE1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human SAE1 Polyclonal Antibody (HA855014)

Overview

Images

References

Datasheet

SDS

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