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Anti-Human SCD5 Polyclonal Antibody (HC065014)

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Overview
Catalog No.HC065014
Description
Anti-Human SCD5 Polyclonal Antibody (HC065014) is a rabbit polyclonal antibody detecting Stearoyl-CoA desaturase 5; 1.14.19.1; Acyl-CoA-desaturase 4; HSCD5; Stearoyl-CoA 9-desaturase; Stearoyl-CoA desaturase 2; SCD5; ACOD4; SCD2; SCD4 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human SCD5 (His94-Lys193&Asn239-Ala330).
Target Stearoyl-CoA desaturase 5; 1.14.19.1; Acyl-CoA-desaturase 4; HSCD5; Stearoyl-CoA 9-desaturase; Stearoyl-CoA desaturase 2; SCD5; ACOD4; SCD2; SCD4
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q86SK9
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Stearoyl-CoA desaturase 5 (SCD5) is a ~37 kDa protein. Stearoyl-CoA desaturase that utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates. Catalyzes the insertion of a cis double bond at the delta-9 position into fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Gives rise to a mixture of 16:1 and 18:1 unsaturated fatty acids. Involved in neuronal cell proliferation and differentiation through down-regulation of EGFR/AKT/MAPK and Wnt signaling pathways.

1. Zhang, S. et al. (2005) The Biochemical journal 388, 135-42. PMID: 15610069
2. Wang, J. et al. (2005) Biochemical and biophysical research communications 332, 735-42. PMID: 15907797
3. Sinner, DI. et al. (2012) PloS one 7, e39787. PMID: 22745828
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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