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Anti-Human SDCCAG8 Polyclonal Antibody (HA350014)

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Overview
Catalog No.HA350014
Description
Anti-Human SDCCAG8 Polyclonal Antibody (HA350014) is a rabbit polyclonal antibody detecting SDCCAG8 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human SDCCAG8 (Glu348-Leu680).
Target Serologically defined colon cancer antigen 8, Antigen NY-CO-8, Centrosomal colon cancer autoantigen protein, hCCCAP, SDCCAG8, CCCAP, NPHP10, HSPC085
Purification Purified by antigen affinity column.
Accession Q86SQ7
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Serologically defined colon cancer antigen 8 (SDCCAG8) is a ~82 kDa protein. Plays a role in the establishment of cell polarity and epithelial lumen formation. Also plays an essential role in ciliogenesis and subsequent Hedgehog signaling pathway that requires the presence of intact primary cilia for pathway activation. Mechanistically, interacts with and mediates RABEP2 centrosomal localization which is critical for ciliogenesis.

1. Airik, R. et al. (2016) PloS one 11, e0156081. PMID: 27224062
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human SDCCAG8 Polyclonal Antibody

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