Anti-Human SDHB Polyclonal Antibody (HB260014)

Name: Anti-Human SDHB Polyclonal Antibody
Brand: abinScience
SKU: HB260014
Price: 96 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB260014

Description

Anti-Human SDHB Polyclonal Antibody (HB260014) is a rabbit polyclonal antibody detecting SDHB in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Danio rerio.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SDHB (Arg38-Val280).

Target

Ip, SDH1, SDHB, Iron-sulfur subunit of complex II, Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, SDH

Purification

Purified by antigen affinity column.

Accession

P21912

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDHB) is a ~31 kDa protein. Iron-sulfur protein (IP) subunit of the succinate dehydrogenase complex (mitochondrial respiratory chain complex II), responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate. Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate.

1. Ardissone, A. et al. (2015) Molecular genetics and metabolism reports 5, 51-54. PMID: 26925370

2. Grønborg, S. et al. (2017) JIMD reports 33, 69-77. PMID: 27604842

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-SDHB polyclonal antibody at 1μg/ml on various samples.
Lane 1: K562 cell lysate
Lane 2: Mouse brain lysate
Lane 3: Mouse heart lysate
Lane 4: Mouse liver lysate
Lane 5: Rat brain lysate
Lane 6: Rat heart lysate
Lane 7: Rat liver lysate

References

Datasheet

Anti-Human SDHB Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human SDHB Polyclonal Antibody (HB260014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us