Anti-Human SEC13 Polyclonal Antibody (HX854014)

Name: Anti-Human SEC13 Polyclonal Antibody
Brand: abinScience
SKU: HX854014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HX854014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human SEC13 (Met15-Gln322).

Target

D3S1231E, SEC13L1, SEC13, SEC13-like protein 1, SEC13-related protein, Protein SEC13 homolog, SEC13R, GATOR complex protein SEC13, SEC13A

Purification

Purified by antigen affinity column.

Accession

P55735

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Protein SEC13 homolog is a ~35 kDa protein. Functions as a component of the nuclear pore complex (NPC) and the COPII coat. At the endoplasmic reticulum, SEC13 is involved in the biogenesis of COPII-coated vesicles. Required for the exit of adipsin (CFD/ADN), an adipocyte-secreted protein from the endoplasmic reticulum.

1. Tang, BL. et al. (1997) Molecular and cellular biology 17, 256-66. PMID: 8972206

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-SEC13 polyclonal antibody at 1μg/ml on various samples.
Lane 1: RAW264.7 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Hela cell lysate
Lane 4: U87 cell lysate
Lane 5: 293T cell lysate
Lane 6: MCF-7 cell lysate
Lane 7: Mouse kidney lysate
Lane 8: Mouse liver lysate

References

Datasheet

Anti-Human SEC13 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human SEC13 Polyclonal Antibody (HX854014)

Overview

Images

References

Datasheet

SDS

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