Anti-Human SELENON Polyclonal Antibody (HP418014)

Name: Anti-Human SELENON Polyclonal Antibody
Brand: abinScience
SKU: HP418014
Price: 96 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HP418014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SELENON (Ser464-Pro590).

Target

SelN, Selenoprotein N, SELENON, SELN, SEPN1

Purification

Purified by antigen affinity column.

Accession

Q9NZV5

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Selenoprotein N (SELENON) is a ~65 kDa protein. Plays an important role in cell protection against oxidative stress and in the regulation of redox-related calcium homeostasis. Regulates the calcium level of the ER by protecting the calcium pump ATP2A2 against the oxidoreductase ERO1A-mediated oxidative damage. Within the ER, ERO1A activity increases the concentration of H(2)O(2), which attacks the luminal thiols in ATP2A2 and thus leads to cysteinyl sulfenic acid formation (-SOH) and SEPN1 reduces the SOH back to free thiol (-SH), thus restoring ATP2A2 activity. Acts as a modulator of ryanodine receptor (RyR) activity: protects RyR from oxidation due to increased oxidative stress, or directly controls the RyR redox state, regulating the RyR-mediated calcium mobilization required for normal muscle development and differentiation.

1. Marino, M. et al. (2015) Human molecular genetics 24, 1843-55. PMID: 25452428

2. Jurynec, MJ. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 12485-90. PMID: 18713863

3. Arbogast, S. et al. (2009) Annals of neurology 65, 677-86. PMID: 19557870

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human SELENON polyclonal antibody at 1ug/mL on recombinant Human SELENON (Catalog No. HP418012).
Western blot

Lane 1: NIH3T3 cell lysate

References

Datasheet

Anti-Human SELENON Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human SELENON Polyclonal Antibody (HP418014)

Overview

Images

References

Datasheet

SDS

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