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Anti-Human SEMA4F Polyclonal Antibody (HC978014)

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Anti-Human SEMA4F Polyclonal Antibody
Anti-Human SEMA4F Polyclonal Antibody
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  • Datasheet

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Overview
Catalog No.HC978014
Description
Anti-Human SEMA4F Polyclonal Antibody (HC978014) is a rabbit polyclonal antibody detecting SEMA4F. Suitable for Human, Rat, and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human SEMA4F (Val59-Thr510).
Target SEMA4F, SEMAM, SEMAW, Sema M, Sema W, Semaphorin-4F, Semaphorin-M, Semaphorin-W
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O95754
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Semaphorin-4F (SEMA4F) is a ~83 kDa protein. Probable cell surface receptor that regulates oligodendroglial precursor cell migration. Might also regulate differentiation of oligodendroglial precursor cells. Has growth cone collapse activity against retinal ganglion-cell axons.

Note For research use only
Images
  • Anti-Human SEMA4F Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-SEMA4F polyclonal antibody at 1ug/mL on various samples.
    Lane 1: HepG2 cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human SEMA4F Polyclonal Antibody

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