Anti-Human SETMAR IP Recombinant Antibody (SAA0224) (HS762013)

Name: Anti-Human SETMAR IP Recombinant Antibody (SAA0224)
Brand: abinScience
SKU: HS762013
Price: 191 USD
Availability: InStock

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50ug
100ug
1mg

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HS762013

Species reactivity

Human

Applications

ELISA, IF, IP

Host species

Mouse

Isotype

IgG2a

Clone ID

SAA0224

Clonality

Monoclonal

Target

Histone-lysine N-methyltransferase SETMAR, SETMAR, SET domain and mariner transposase fusion protein, Metnase

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q53H47

RRID

Anti-Human SETMAR IP Antibody (SAA0224) (abinScience Cat# HS762013, RRID:AB_3729758)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Histone-lysine N-methyltransferase SETMAR is a ~78 kDa protein. Protein derived from the fusion of a methylase with the transposase of an Hsmar1 transposon that plays a role in DNA double-strand break repair, stalled replication fork restart and DNA integration. DNA-binding protein, it is indirectly recruited to sites of DNA damage through protein-protein interactions. Also has kept a sequence-specific DNA-binding activity recognizing the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element and displays a DNA nicking and end joining activity. In parallel, has a histone methyltransferase activity and methylates 'Lys-4' and 'Lys-36' of histone H3. Specifically mediates dimethylation of H3 'Lys-36' at sites of DNA double-strand break and may recruit proteins required for efficient DSB repair through non-homologous end-joining.

1. Lee, SH. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 18075-80. PMID: 16332963

2. Cordaux, R. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 8101-6. PMID: 16672366

3. Miskey, C. et al. (2007) Molecular and cellular biology 27, 4589-600. PMID: 17403897

4. Roman, Y. et al. (2007) Biochemistry 46, 11369-76. PMID: 17877369

5. Beck, BD. et al. (2008) The Journal of biological chemistry 283, 9023-30. PMID: 18263876

6. Goodwin, KD. et al. (2010) Biochemistry 49, 5705-13. PMID: 20521842

7. Hromas, R. et al. (2012) Oncogene 31, 4245-54. PMID: 22231448

8. Kim, HS. et al. (2014) The Journal of biological chemistry 289, 10930-10938. PMID: 24573677

9. Fnu, S. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 540-5. PMID: 21187428

10. Williamson, EA. et al. (2008) Nucleic acids research 36, 5822-31. PMID: 18790802

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human SETMAR IP Antibody (SAA0224)

References

Datasheet

Anti-Human SETMAR IP Recombinant Antibody (SAA0224).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human SETMAR IP Recombinant Antibody (SAA0224) (HS762013)

Overview

Images

References

Datasheet

SDS

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