Anti-Human SF3B3 Polyclonal Antibody (HS767014)

Name: Anti-Human SF3B3 Polyclonal Antibody
Brand: abinScience
SKU: HS767014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HS767014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SF3B3 (Met1-Ala362).

Target

STAF130, KIAA0017, SF3B3, Splicing factor 3B subunit 3, SF3b130, Pre-mRNA-splicing factor SF3b 130 kDa subunit, SAP130, SAP 130, Spliceosome-associated protein 130

Purification

Purified by antigen affinity column.

Accession

Q15393

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Splicing factor 3B subunit 3 (SF3B3) is a ~135 kDa protein. Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3B3 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.

1. Das, BK. et al. (1999) Molecular and cellular biology 19, 6796-802. PMID: 10490618

2. Das, R. et al. (2000) Molecular cell 5, 779-87. PMID: 10882114

3. Will, CL. et al. (2002) The EMBO journal 21, 4978-88. PMID: 12234937

4. Cretu, C. et al. (2016) Molecular cell 64, 307-319. PMID: 27720643

5. Bertram, K. et al. (2017) Cell 170, 701-713.e11. PMID: 28781166

6. Zhang, Z. et al. (2020) Nature 583, 310-313. PMID: 32494006

7. Tholen, J. et al. (2022) Science (New York, N.Y.) 375, 50-57. PMID: 34822310

Note

For research use only.

Images

References

Datasheet

Anti-Human SF3B3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human SF3B3 Polyclonal Antibody (HS767014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us