Anti-Human SFPQ Polyclonal Antibody (HB205014)

Name: Anti-Human SFPQ Polyclonal Antibody
Brand: abinScience
SKU: HB205014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB205014

Description

Anti-Human SFPQ Polyclonal Antibody (HB205014) is a rabbit polyclonal antibody detecting SFPQ in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SFPQ (Lys271-Gly434).

Target

Splicing factor, proline- and glutamine-rich, PTB-associated-splicing factor, SFPQ, DNA-binding p52/p100 complex, 100 kDa subunit, Polypyrimidine tract-binding protein-associated-splicing factor, PSF, hPOMp100, 100 kDa DNA-pairing protein

Purification

Purified by antigen affinity column.

Accession

P23246

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Splicing factor, proline- and glutamine-rich (SFPQ) is a ~76 kDa protein. DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45, a phosphorylated form is sequestered by THRAP3 from the pre-mRNA in resting T-cells; T-cell activation and subsequent reduced phosphorylation is proposed to lead to release from THRAP3 allowing binding to pre-mRNA splicing regulatotry elements which represses exon inclusion.

1. Lee, M. et al. (2015) Nucleic acids research 43, 3826-40. PMID: 25765647

2. Morchikh, M. et al. (2017) Molecular cell 67, 387-399.e5. PMID: 28712728

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-SFPQ polyclonal antibody at 1μg/ml on various samples.
Lane 1: HepG2 cell lysate
Lane 2: Rat brain lysate
Lane 3: Jurkat cell lysate

References

Datasheet

Anti-Human SFPQ Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human SFPQ Polyclonal Antibody (HB205014)

Overview

Images

References

Datasheet

SDS

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