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Anti-Human SH3BP5 Polyclonal Antibody (HT138014)

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Overview
Catalog No.HT138014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human SH3BP5 (Ser10-Glu276).
Target SAB, SH3 domain-binding protein that preferentially associates with BTK, SH3 domain-binding protein 5, SH3BP5, SH3BP-5
Purification Purified by antigen affinity column.
Accession O60239
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

SH3 domain-binding protein 5 (SH3BP5) is a ~50 kDa protein. Functions as a guanine nucleotide exchange factor (GEF) with specificity for RAB11A and RAB25. Inhibits the auto- and transphosphorylation activity of BTK. Plays a negative regulatory role in BTK-related cytoplasmic signaling in B-cells. May be involved in BCR-induced apoptotic cell death.

1. Sakaguchi, A. et al. (2015) Developmental cell 35, 211-21. PMID: 26506309
2. Jenkins, ML. et al. (2018) Nature communications 9, 3772. PMID: 30217979
Note For research use only.
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Anti-Human SH3BP5 Polyclonal Antibody

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