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Anti-Human SHC3 Polyclonal Antibody (HV158014)

Anti-Human SHC3 Polyclonal Antibody
Anti-Human SHC3 Polyclonal Antibody
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Overview
Catalog No.HV158014
Description
Anti-Human SHC3 Polyclonal Antibody (HV158014) is a rabbit polyclonal antibody detecting SHC3. Suitable for Rat, Human, and Mouse.
Highlights
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human SHC3 (Ser145-Gln310).
Target N-Shc, NSHC, Neuronal Shc, Protein Rai, SH2 domain protein C3, SHC-transforming protein 3, SHC-transforming protein C, SHC3, SHCC, Src homology 2 domain-containing-transforming protein C3
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q92529
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

SHC-transforming protein 3 (SHC3) is a ~64 kDa protein. Signaling adapter that couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons.

Note For research use only
Images
  • Anti-Human SHC3 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-SHC3 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: BxPC-3 cell lysate
    Lane 2: A549 cell lysate
    Lane 3: NIH3T3 cell lysate
    Lane 4: HepG2 cell lysate
    Lane 5: SH-SY5Y cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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