Anti-Human SIRT3 Polyclonal Antibody (HP409014)

Name: Anti-Human SIRT3 Polyclonal Antibody
Brand: abinScience
SKU: HP409014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HP409014

Description

Anti-Human SIRT3 Polyclonal Antibody (HP409014) is a rabbit polyclonal antibody detecting SIRT3 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human SIRT3 (Lys122-Lys399).

Target

NAD-dependent protein deacetylase sirtuin-3, mitochondrial, SIR2-like protein 3, SIRT3, Regulatory protein SIR2 homolog 3, SIR2L3, hSIRT3

Purification

Purified by antigen affinity column.

Accession

Q9NTG7

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

NAD-dependent protein deacetylase sirtuin-3, mitochondrial (SIRT3) is a ~43 kDa protein. NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA, MRPL12 and the ATP synthase subunit ATP5PO. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.

1. Schwer, B. et al. (2002) The Journal of cell biology 158, 647-57. PMID: 12186850

2. Onyango, P. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 13653-8. PMID: 12374852

3. Schwer, B. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 10224-10229. PMID: 16788062

4. Schlicker, C. et al. (2008) Journal of molecular biology 382, 790-801. PMID: 18680753

5. Ahn, BH. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 14447-52. PMID: 18794531

6. Jin, L. et al. (2009) The Journal of biological chemistry 284, 24394-405. PMID: 19535340

7. Peserico, A. et al. (2013) Cellular and molecular life sciences : CMLS 70, 2015-29. PMID: 23283301

8. Bharathi, SS. et al. (2013) The Journal of biological chemistry 288, 33837-33847. PMID: 24121500

9. Vassilopoulos, A. et al. (2014) Antioxidants & redox signaling 21, 551-64. PMID: 24252090

Note

For research use only.

Images

References

Datasheet

Anti-Human SIRT3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human SIRT3 Polyclonal Antibody (HP409014)

Overview

Images

References

Datasheet

SDS

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