Anti-Human SIX1 Polyclonal Antibody (HA515014)

Name: Anti-Human SIX1 Polyclonal Antibody
Brand: abinScience
SKU: HA515014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HA515014

Description

Anti-Human SIX1 Polyclonal Antibody (HA515014) is a rabbit polyclonal antibody detecting SIX1 in ELISA, IHC, WB. Suitable for Mus musculus, Danio rerio, Human, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SIX1 (Met1-Lys199).

Target

SIX1

Purification

Purified by antigen affinity column.

Accession

Q15475

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Homeobox protein SIX1, Sine oculis homeobox homolog 1, SIX1

Background

Homeobox protein SIX1 is a ~32 kDa protein. Transcription factor that is involved in the regulation of cell proliferation, apoptosis and embryonic development. Plays an important role in the development of several organs, including kidney, muscle and inner ear. Depending on context, functions as a transcriptional repressor or activator. Lacks an activation domain, and requires interaction with EYA family members for transcription activation. Mediates nuclear translocation of EYA1 and EYA2.

1. Ruf, RG. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 8090-5. PMID: 15141091

2. Patrick, AN. et al. (2009) The Journal of biological chemistry 284, 20781-90. PMID: 19497856

3. Patrick, AN. et al. (2013) Nature structural & molecular biology 20, 447-53. PMID: 23435380

4. Brunmeir, R. et al. (2016) PLoS genetics 12, e1006474. PMID: 27923061

5. Coletta, RD. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 6478-83. PMID: 15123840

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-SIX1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse skeletal muscle lysate

References

Datasheet

Anti-Human SIX1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human SIX1 Polyclonal Antibody (HA515014)

Overview

Images

References

Datasheet

SDS

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