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Anti-Human SLC2A5 Polyclonal Antibody (HB050014)

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Overview
Catalog No.HB050014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human SLC2A5 (Pro461-Gln501).
Target SLC2A5, GLUT-5, Glucose transporter type 5, small intestine, GLUT5, Solute carrier family 2, facilitated glucose transporter member 5, Fructose transporter
Purification Purified by antigen affinity column.
Accession P22732
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Solute carrier family 2, facilitated glucose transporter member 5 (SLC2A5) is a ~54 kDa protein. Functions as a fructose transporter that has only low activity with other monosaccharides. Can mediate the uptake of 2-deoxyglucose, but with low efficiency. Essential for fructose uptake in the small intestine. Plays a role in the regulation of salt uptake and blood pressure in response to dietary fructose. Required for the development of high blood pressure in response to high dietary fructose intake.

1. Manolescu, A. et al. (2005) The Journal of biological chemistry 280, 42978-83. PMID: 16186102
2. Manolescu, AR. et al. (2007) Molecular membrane biology 24, 455-63. PMID: 17710649
3. Ebert, K. et al. (2017) The Journal of membrane biology 250, 171-182. PMID: 28083649
4. Gauer, JS. et al. (2018) Biochemical pharmacology 152, 11-20. PMID: 29548810
5. Rand, EB. et al. (1993) The American journal of physiology 264, G1169-76. PMID: 8333543
6. Kayano, T. et al. (1990) The Journal of biological chemistry 265, 13276-82. PMID: 1695905
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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