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Anti-Human SLC9A9 Polyclonal Antibody (HA176014)

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Overview
Catalog No.HA176014
Description
Anti-Human SLC9A9 Polyclonal Antibody (HA176014) is a rabbit polyclonal antibody detecting SLC9A9 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human SLC9A9.
Target Sodium/hydrogen exchanger 9, Na(+)/H(+) exchanger 9, NHE-9, Solute carrier family 9 member 9, SLC9A9, NHE9, Nbla00118
Purification Purified by antigen affinity column.
Accession Q8IVB4
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Sodium/hydrogen exchanger 9 (SLC9A9) is a ~72 kDa protein. Endosomal Na(+), K(+)/H(+) antiporter. Mediates the electroneutral exchange of endosomal luminal H(+) for a cytosolic Na(+) or K(+) (Probable). By facilitating proton efflux, SLC9A9 counteracts the acidity generated by vacuolar (V)-ATPase, thereby limiting luminal acidification. Regulates organellar pH and consequently, e.g., endosome maturation and endocytic trafficking of plasma membrane receptors and neurotransporters. Promotes the recycling of transferrin receptors back to the cell surface to facilitate additional iron uptake in the brain.

1. Nakamura, N. et al. (2005) The Journal of biological chemistry 280, 1561-72. PMID: 15522866
2. Kondapalli, KC. et al. (2013) Nature communications 4, 2510. PMID: 24065030
3. Beydoun, R. et al. (2017) The Journal of biological chemistry 292, 4293-4301. PMID: 28130443
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human SLC9A9 Polyclonal Antibody

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