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Anti-Human SMARCD1 Polyclonal Antibody (HP029014)

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Overview
Catalog No.HP029014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human SMARCD1 (Lys177-Ser410).
Target 60 kDa BRG-1/Brm-associated factor subunit A, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1, SWI/SNF complex 60 kDa subunit, BAF60A, SMARCD1, BRG1-associated factor 60A
Purification Purified by antigen affinity column.
Accession Q96GM5
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 (SMARCD1) is a ~58 kDa protein. Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes.

1. Alpsoy, A. et al. (2018) The Journal of biological chemistry 293, 3892-3903. PMID: 29374058
2. Wang, W. et al. (1996) Genes & development 10, 2117-30. PMID: 8804307
3. Koszewski, NJ. et al. (2003) The Journal of steroid biochemistry and molecular biology 87, 223-31. PMID: 14698202
4. Hsiao, PW. et al. (2003) Molecular and cellular biology 23, 6210-20. PMID: 12917342
Note For research use only.
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Formula
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Anti-Human SMARCD1 Polyclonal Antibody

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