Anti-Human SMO/Smoothened Recombinant Antibody (SAA1978) (HV714013)

Name: Anti-Human SMO/Smoothened Recombinant Antibody (SAA1978)
Brand: abinScience
SKU: HV714013
Price: 196 USD
Availability: InStock

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100ug
1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HV714013

Species reactivity

Human

Applications

ELISA

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

SAA1978

Clonality

Monoclonal

Target

SMOH, Smoothened homolog, SMO, Protein Gx

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q99835

RRID

Anti-Human SMO/Smoothened Antibody (SAA1978) (abinScience Cat# HV714013, RRID:AB_3727742)
_x005f_x000D_

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Protein smoothened is a ~86 kDa protein. G protein-coupled receptor which associates with the patched protein (PTCH) to transduce hedgehog protein signaling. Binding of sonic hedgehog (SHH) to its receptor patched prevents inhibition of smoothened (SMO) by patched. When active, SMO binds to and sequesters protein kinase A catalytic subunit PRKACA at the cell membrane, preventing PRKACA-mediated phosphorylation of GLI transcription factors which releases the GLI proteins from PRKACA-mediated inhibition and allows for transcriptional activation of hedgehog pathway target genes. Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia. Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation.

1. Endoh-Yamagami, S. et al. (2009) Current biology : CB 19, 1320-6. PMID: 19592253

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human SMO/Smoothened Antibody (SAA1978)

References

Datasheet

Anti-Human SMO/Smoothened Recombinant Antibody (SAA1978).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human SMO/Smoothened Recombinant Antibody (SAA1978) (HV714013)

Overview

Images

References

Datasheet

SDS

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