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Anti-Human SMPD2 Polyclonal Antibody (HF988014)

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Overview
Catalog No.HF988014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human SMPD2 (Met1-Arg236).
Target Neutral sphingomyelinase, Lyso-PAF-PLC, SMPD2, Sphingomyelin phosphodiesterase 2, nSMase, Lyso-platelet-activating factor-phospholipase C, nSMase1, N-SMase
Purification Purified by antigen affinity column.
Accession O60906
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Sphingomyelin phosphodiesterase 2 (SMPD2) is a ~47 kDa protein. Catalyzes, at least in vitro, the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Also hydrolyzes 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor) in vivo. Also acts on 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and sphingosylphosphocholine.

1. Sawai, H. et al. (1999) The Journal of biological chemistry 274, 38131-9. PMID: 10608884
2. Miura, Y. et al. (2004) FEBS letters 557, 288-92. PMID: 14741383
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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