Anti-Human SMURF2 Polyclonal Antibody (HV523014)

Name: Anti-Human SMURF2 Polyclonal Antibody
Brand: abinScience
SKU: HV523014
Price: 96 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HV523014

Description

Anti-Human SMURF2 Polyclonal Antibody (HV523014) is a rabbit polyclonal antibody detecting SMURF2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SMURF2 (His246-Glu400).

Target

SMAD ubiquitination regulatory factor 2, hSMURF2, HECT-type E3 ubiquitin transferase SMURF2, SMURF2, SMAD-specific E3 ubiquitin-protein ligase 2, E3 ubiquitin-protein ligase SMURF2

Purification

Purified by antigen affinity column.

Accession

Q9HAU4

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

E3 ubiquitin-protein ligase SMURF2 is a ~86 kDa protein. E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD7 to trigger SMAD7-mediated transforming growth factor beta/TGF-beta receptor ubiquitin-dependent degradation, thereby down-regulating TGF-beta signaling. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with AIMP1. Also forms a stable complex with TGF-beta receptor-mediated phosphorylated SMAD1, SMAD2 and SMAD3, and targets SMAD1 and SMAD2 for ubiquitination and proteasome-mediated degradation. SMAD2 may recruit substrates, such as SNON, for ubiquitin-dependent degradation.

1. Lin, X. et al. (2000) The Journal of biological chemistry 275, 36818-22. PMID: 11016919

2. Zhang, W. et al. (2023) Molecular cell 83, 4352-4369.e8. PMID: 38016474

3. Kavsak, P. et al. (2000) Molecular cell 6, 1365-75. PMID: 11163210

4. Di Guglielmo, GM. et al. (2003) Nature cell biology 5, 410-21. PMID: 12717440

5. Yan, X. et al. (2011) Molecular and cellular biology 31, 3700-9. PMID: 21791611

6. Lee, YS. et al. (2008) Biochemical and biophysical research communications 371, 395-400. PMID: 18448069

7. Zhang, Y. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 974-9. PMID: 11158580

8. Bonni, S. et al. (2001) Nature cell biology 3, 587-95. PMID: 11389444

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-SMURF2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: MDA-MB-231 cell lysate

References

Datasheet

Anti-Human SMURF2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human SMURF2 Polyclonal Antibody (HV523014)

Overview

Images

References

Datasheet

SDS

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