Anti-Human SPAG5 Polyclonal Antibody (HP190014)

Name: Anti-Human SPAG5 Polyclonal Antibody
Brand: abinScience
SKU: HP190014
Price: 96 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HP190014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SPAG5 (Met976-Asp1098).

Target

Sperm-associated antigen 5, SPAG5, Astrin, Deepest, MAP126, Mitotic spindle-associated protein p126

Purification

Purified by antigen affinity column.

Accession

Q96R06

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Sperm-associated antigen 5 (SPAG5) is a ~134 kDa protein. Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes.

1. Mack, GJ. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 14434-9. PMID: 11724960

2. Gruber, J. et al. (2002) Journal of cell science 115, 4053-9. PMID: 12356910

3. Chu, X. et al. (2016) The Journal of biological chemistry 291, 20055-67. PMID: 27462074

4. Thein, KH. et al. (2007) The Journal of cell biology 178, 345-54. PMID: 17664331

5. Du, J. et al. (2008) Biochemical and biophysical research communications 370, 213-9. PMID: 18361916

6. Dunsch, AK. et al. (2011) The Journal of cell biology 192, 959-68. PMID: 21402792

7. Kodani, A. et al. (2015) eLife 4. PMID: 26297806

8. Thedieck, K. et al. (2013) Cell 154, 859-74. PMID: 23953116

9. Cheng, TS. et al. (2008) The Journal of biological chemistry 283, 2454-64. PMID: 18055457

Note

For research use only.

Images

References

Datasheet

Anti-Human SPAG5 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human SPAG5 Polyclonal Antibody (HP190014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us