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Anti-Human SPARC/Osteonectin Recombinant Antibody (SAA1953) (HY111013)

Anti-Human SPARC/Osteonectin Recombinant Antibody (SAA1953)
Anti-Human SPARC/Osteonectin Recombinant Antibody (SAA1953)
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Overview
Catalog No.HY111013
Species reactivityHuman
ApplicationsELISA
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDSAA1953
Clonality Monoclonal
Target Secreted protein acidic and rich in cysteine, Basement-membrane protein 40, BM-40, Osteonectin, ON, SPARC
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P09486
RRID

Anti-Human SPARC/Osteonectin Antibody (SAA1953) (abinScience Cat# HY111013, RRID:AB_3727717)
_x005F_x000D_

Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

SPARC (Osteonectin) is a ~34 kDa protein. Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Note For research use only.
Images
  • Anti-Human SPARC/Osteonectin Recombinant Antibody (SAA1953)

    SDS-PAGE

    SDS-PAGE for Anti-Human SPARC/Osteonectin Antibody (SAA1953)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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