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Anti-Human SPART Polyclonal Antibody (HC761014)

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Anti-Human SPART Polyclonal Antibody
Anti-Human SPART Polyclonal Antibody
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Overview
Catalog No.HC761014
Description
Anti-Human SPART Polyclonal Antibody (HC761014) is a rabbit polyclonal antibody detecting SPART. Suitable for Mouse and Human.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human SPART (Gly198-Gln336).
Target KIAA0610, SPART, SPG20, Spartin, Spastic paraplegia 20 protein, TAHCCP1, Trans-activated by hepatitis C virus core protein 1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q8N0X7
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Spartin is a ~72 kDa protein. Lipophagy receptor that plays an important role in lipid droplet (LD) turnover in motor neurons. Localizes to LDs and interacts with components of the autophagy machinery, such as MAP1LC3A/C proteins to deliver LDs to autophagosomes for degradation via lipophagy. Lipid transfer protein required for lipid droplet degradation, including by lipophagy. Can bind and transfer all lipid species found in lipid droplets, from phospholipids to triglycerides and sterol esters but the direction of lipid transfer by spartin and its cargos are unknown. May be implicated in endosomal trafficking, or microtubule dynamics, or both.

1. Chung, J. et al. (2023) Nature cell biology 25, 1101-1110. PMID: 37443287
2. Wan, N. et al. (2024) Proceedings of the National Academy of Sciences of the United States of America 121, e2314093121. PMID: 38190532
3. Renvoisé, B. et al. (2010) Molecular biology of the cell 21, 3293-303. PMID: 20719964
Note For research use only
Images
  • Anti-Human SPART Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-SPART polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: PANC1 cell lysate
    Lane 3: HL-60 cell lysate
    Lane 4: MDA-MB-231 cell lysate
    Lane 5: K562 cell lysate
    Lane 6: 293T cell lysate
    Lane 7: Mouse heart lysate
    Lane 8: Rat heart lysate
    Lane 9: Mouse brain lysate
    Lane 10: Rat brain lysate
    Lane11: Mouse spleen lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human SPART Polyclonal Antibody

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