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Anti-Human SULT1C2 Polyclonal Antibody (HT423014)

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Overview
Catalog No.HT423014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human SULT1C2 (Lys12-Leu296).
Target SULT1C2, humSULTC2, Sulfotransferase 1C1, ST1C2, SULT1C#1, SULT1C1, Sulfotransferase 1C2
Purification Purified by antigen affinity column.
Accession O00338
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Sulfotransferase 1C2 (SULT1C2) is a ~34 kDa protein. Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the sulfate conjugation of phenolic compounds. Does not transfer sulfate to steroids, dopamine, acetaminophen, or alpha-naphthol. Except in mitochondria, where it can add sulfate to cholesterol producing cholesterol sulfate, which alters mitochondrial membrane organization, and impacts protein complex mobility increasing state-III respiration, thereby modulating mitochondrial respiration. Catalyzes the sulfation of the carcinogenic N-hydroxy-2-acetylaminofluorene leading to highly reactive intermediates capable of forming DNA adducts, potentially resulting in mutagenesis.

1. Hehonah, N. et al. (1999) The international journal of biochemistry & cell biology 31, 869-82. PMID: 10481272
2. Freimuth, RR. et al. (2000) Genomics 65, 157-65. PMID: 10783263
3. Sakakibara, Y. et al. (1998) The Journal of biological chemistry 273, 33929-35. PMID: 9852044
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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