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Anti-Human SVIL Polyclonal Antibody (HF800014)

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Overview
Catalog No.HF800014
Description
Anti-Human SVIL Polyclonal Antibody (HF800014) is a rabbit polyclonal antibody detecting SVIL. Suitable for Mouse and Human.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human SVIL (Met1444-Lys1678).
Target Archvillin, SVIL, Supervillin, p205/p250
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O95425
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Supervillin (SVIL) is a ~247 kDa protein. Forms a high-affinity link between the actin cytoskeleton and the membrane. Is among the first costameric proteins to assemble during myogenesis and it contributes to myogenic membrane structure and differentiation. Appears to be involved in myosin II assembly. May modulate myosin II regulation through MLCK during cell spreading, an initial step in cell migration. May play a role in invadopodial function.

1. Oh, SW. et al. (2003) Journal of cell science 116, 2261-75. PMID: 12711699
2. Crowley, JL. et al. (2009) Molecular biology of the cell 20, 948-62. PMID: 19109420
3. Laurent, B. et al. (2015) Molecular cell 57, 957-970. PMID: 25684206
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human SVIL Polyclonal Antibody

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